Endothelial Transwell Permeability Assay Kit (24 SPL PC inserts, 100 assays)

Introduction

The microvascular endothelial cell monolayer localized at the critical interface between the blood and vessel wall has the vital functions of regulating tissue fluid balance and supplying the essential nutrients needed for the survival of the organism. The endothelial cells are able to dynamically regulate its paracellular and transcellular pathways for transport of plasma proteins, solutes, and liquid. The endothelial barrier is a well-regulated structure, which maintains a minimal and selective permeability to fluid and molecules under normal physiological conditions. Vascular leakage is an important feature in several diseases, such as septic shock, inflammation, disruption of blood-brain barrier (BBB)-related neurological disorders, viral hemorrhagic fever, cancer and ischemia-reperfusion injuries. It is emergent to develop a highly sensitive and reliable assay to measure endothelial permeability.

A simple, reproducible, sensitive, quantitative transwell permeability assay is developed to measure endothelial barrier function for wide analyses of multiple types of endothelial permeability in vitro. The assay will be performed by a spectrometer-based absorbance reader (ELISA plate reader) at 450 nm within one hour post endothelial treatment. The assay kit is suitable for primary endothelial cells and immortal cell lines from multiple species.

For more information, please see Related Documents CB6929 PDF.